Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16389
Title: Role of Substance P in Sepsis
Authors: AKHIL KUMAR HEGDE RAMA
Keywords: sepsis, substance P, preprotachykinin-A, mice, microarray, neurokinin receptor
Issue Date: 23-Jun-2009
Source: AKHIL KUMAR HEGDE RAMA (2009-06-23). Role of Substance P in Sepsis. ScholarBank@NUS Repository.
Abstract: The present study evaluates the role of substance P (SP) in polymicrobial-sepsis associated lung injury. Preprotachykinin-A gene knock-out and wild type mice were subject to sham or cecal ligation and puncture surgery. Affymetrix high-density oligonucleotide arrays were used for lung gene expression profiling. Genes that were either consistently increased or decreased were shortlisted and RT-PCR and ELISA were used to validate the results for selected differentially expressed genes. In addition to the use of gene knock-out mouse model, pharmacological blocking of the receptors with SR140333, a highly potent and selective antagonist of the neurokinin-1 (NK-1) receptors, and GR159897, a neurokinin-2 (NK-2) receptor antagonist, were also studied. 8 h after surgery, lung tissue was collected and analyzed for myeloperoxidase activity, histology, chemokines, cytokines and adhesion molecules. Transcription factors NF-N:B and AP-1 were analysed to elucidate the mechanism involved. The results provide further support for the role of SP in polymicrobial sepsis.
URI: http://scholarbank.nus.edu.sg/handle/10635/16389
Appears in Collections:Ph.D Theses (Open)

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