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Title: The effects of fungal stress on the selected plant seeds and its applications for novel food development
Keywords: fungal stress, black soybean seed, phytoalexins, yogurt, durian seed
Issue Date: 31-Mar-2009
Citation: FENG SHENGBAO (2009-03-31). The effects of fungal stress on the selected plant seeds and its applications for novel food development. ScholarBank@NUS Repository.
Abstract: The aim of this research is to study the effects of fungal stress on plant seeds and its applications for novel food development. We discovered that microbial-stressed germination of black soybean seeds led to the generation of a group of oxylipins, oxooctadecadienoic acids, and their respective glyceryl esters in addition to glyceollins, a known group of phytoalexins present in wild and pathogen infected germinating beans. Different microbes had different efficiency of inducing these compounds. We further investigated the antioxidant capacities of the germinating beans after R. oligosporus - induced stress. Results showed that the antioxidant capacity remained high after fungal stress. The fungal stressed and germinated black soybeans were further processed for functional food development. We modified the traditional procedures of soy yogurt making using these treated beans. The satisfied lactic acid bacterial (LAB) growth in the soymilk demonstrated that the fungal stressed soybean milk was an optimum medium for LAB fermentation. Importantly, the novel process had reduced flatulence - causing oligosaccharides to negligible contents. In addition, the soy yogurt had significantly altered the micronutrient profiles resulting in enriched glyceollins, which are known to have anti-cancer properties. We also inoculated fungi to the germinating durian seeds in a purpose of exploring the generation of functional phytoalexins from other seeds particularly from food waste. We discovered the content of oligomeric proanthocyanidins (OPCs) in durian seeds for the first time. After stressing the germinating durian seeds with food grade R. oligosporus, new phytoalexins were detected but with low concentrations.
Appears in Collections:Ph.D Theses (Open)

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