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Title: Knitted scaffold reinforced fibroblast cell-sheet for ligament and tendon tissue engineering
Authors: ZHENG YE
Keywords: fibroblast cell sheet; ascorbic acid; ligament and tendon tissue engineering; knitted silk scaffold; bioreactor
Issue Date: 8-Jul-2009
Citation: ZHENG YE (2009-07-08). Knitted scaffold reinforced fibroblast cell-sheet for ligament and tendon tissue engineering. ScholarBank@NUS Repository.
Abstract: This dissertation describes a method to fabricate fibroblast cell sheet and assemble it onto knitted scaffold for engineering ligament/tendon analogs. Firstly, fibroblast was cultured in various concentrations of FBS and ascorbic acid to obtain optimized culture condition. Once cell sheet was obtained, it was assembled onto knitted scaffolds by wrapping technique. Immunohistochemical analysis showed that cell sheet-scaffold hybrid had transformed into tissue-like analogs and the components of the analogs were similar to that of nature tissue. Moreover, cellular proliferation and function was more actively exhibited on silk sponge modified scaffolds than other scaffolds. Furthermore, when cultured in bioreactor, the cyclic strain promoted cell proliferation and matrix protein expression. Therefore, the approach of assembling fibroblast cell sheet onto silk sponge modified knitted silk scaffold is promising to enhance fibroblast cell sheet and produce tissue-like and functional ligament/tendon analogs. Bioreactor culture efficiently enhances cell function for the purpose of ligament/tendon reconstruction.
Appears in Collections:Ph.D Theses (Open)

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