Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/16311
DC Field | Value | |
---|---|---|
dc.title | The role of NF-kB and histone deacetylase in gene regulation | |
dc.contributor.author | CHEW SOO FEN, JOANNE CHRISTABELLE | |
dc.date.accessioned | 2010-04-08T11:03:25Z | |
dc.date.available | 2010-04-08T11:03:25Z | |
dc.date.issued | 2009-08-10 | |
dc.identifier.citation | CHEW SOO FEN, JOANNE CHRISTABELLE (2009-08-10). The role of NF-kB and histone deacetylase in gene regulation. ScholarBank@NUS Repository. | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/16311 | |
dc.description.abstract | Post-translational modifications of NF-kB via phosphorylations enhance the transactivation potential of NF-kB. Much is known about the kinases that phosphorylate NF-kB, but little is known about the phosphatases that dephosphorylate NF-kB. Here, we report the regulation of NF-kB by the WIP1 phosphatase and its role in inflammation. Overexpression of WIP1 in HeLa cervical cancer and Saos-2 osteoscarcoma cells results in decreased NF-kB activation in a manner dependent on the dosage of WIP1. Overexpression of WIP1 could also repress the expression of endogenous NF-kB target genes in response to inflammatory stimuli. Conversely, knockdown of WIP1 results in increased NF-kB transcriptional function. To investigate the molecular mechanism by which WIP1 regulates NF-kB function, we investigated whether WIP1 can dephosphorylate any component of the NF-kB signaling cascade. Using in vitro and in vivo experiments, we demonstrate that WIP1 is a direct phosphatase on serine 536 of the p65 subunit of NF-kB. | |
dc.language.iso | en | |
dc.subject | role, NF-kB, NF-kappaB, histone deacetylase, HDAC, gene regulation | |
dc.type | Thesis | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.supervisor | VINAY TERGAONKAR | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
The Role of NFKB and Histone Deacetylase in Gene Regulation.pdf | 6.97 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.