Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16230
Title: Genetic study of hematopoiesis in zebrafish - Characterization of zebrafish udu mutant, positional cloning and functional study of udu gene
Authors: LIU YANMEI
Keywords: udu, hematopoiesis, zebrafish, erythroid, positional cloning, cell cycle
Issue Date: 3-May-2007
Source: LIU YANMEI (2007-05-03). Genetic study of hematopoiesis in zebrafish - Characterization of zebrafish udu mutant, positional cloning and functional study of udu gene. ScholarBank@NUS Repository.
Abstract: Hematopoiesis is a complex process which gives rise to all blood lineages in the course of an organism's lifespan. Yet the underlying molecular mechanism governing this process is not fully understood. In this thesis I report the isolation and detailed study of a newly identified zebrafish ugly duckling (udu) mutant allele, udusq1zl. Loss-of-function mutation in udu gene disrupts primitive erythroid cell proliferation and differentiation in a cell-autonomous manner, resulting in red blood cell hypoplasia. Positional cloning reveals that udu gene encodes a novel nuclear factor that contains two Paired Amphipathic a-Helix-like (PAH-L) repeats and a putative SW13, ADA2, N-Cor and TFIIIB-like (SANT-L) domain. Moreover, the expression of the tumor suppressor p53 and several p53 downstream targets is elevated in udusq1zl mutant embryos. Knockdown of p53 protein expression by p53 antisense morpholino oligos could correct the mutant phenotype to the same extent as udu RNA injections in mutant embryos. Thus, these results indicate that the Udu protein is very likely to function as a transcription modulator essential for the proliferation and differentiation of erythroid lineage, probably through a p53-dependent pathway.
URI: http://scholarbank.nus.edu.sg/handle/10635/16230
Appears in Collections:Ph.D Theses (Open)

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