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https://doi.org/10.1371/journal.pone.0041895
Title: | Immunodominance of antigenic site B over site a of hemagglutinin of recent H3N2 influenza viruses | Authors: | Popova L. Smith K. West A.H. Wilson P.C. James J.A. Thompson L.F. Air G.M. |
Keywords: | influenza vaccine Influenza virus hemagglutinin monoclonal antibody neutralizing antibody polyclonal antibody antigen binding antigen recognition antigen structure article binding site blood analysis controlled study enzyme linked immunosorbent assay human immunogenicity immunoreactivity infection prevention influenza A (H3N2) influenza vaccination Influenza virus A H3N2 nonhuman prediction protein expression site directed mutagenesis Antibodies, Monoclonal Antibodies, Neutralizing Antigens, Viral Epitopes Hemagglutinin Glycoproteins, Influenza Virus Humans Influenza A Virus, H3N2 Subtype Models, Molecular Mutation Protein Conformation Seasons Species Specificity Vaccination Orthomyxoviridae |
Issue Date: | 2012 | Citation: | Popova L., Smith K., West A.H., Wilson P.C., James J.A., Thompson L.F., Air G.M. (2012). Immunodominance of antigenic site B over site a of hemagglutinin of recent H3N2 influenza viruses. PLoS ONE 7 (7) : e41895. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0041895 | Rights: | Attribution 4.0 International | Abstract: | H3N2 influenza viruses have now circulated in the human population for 43 years since the pandemic of 1968, accumulating sequence changes in the hemagglutinin (HA) and neuraminidase (NA) that are believed to be predominantly due to selection for escape from antibodies. Examination of mutations that persist and accumulate led to identification of antigenically significant mutations that are contained in five antigenic sites (A-E) mapped on to the H3 HA. In early H3N2 isolates, antigenic site A appeared to be dominant while in the 1990s site B seemed more important. To obtain experimental evidence for dominance of antigenic sites on modern H3 HAs, we have measured antibodies in plasma of human subjects who received the 2006-07 trivalent subunit influenza vaccine (H3 component A/Wisconsin/67/05) or the 2008-09 formulation (H3 component A/Uruguay/716/07). Plasmas were tested against expressed HA of Wisconsin-like influenza A/Oklahoma/309/06 and site-directed mutants in antigenic site A (NNES121-124ITEG, N126T, N133D, TSSS135-138GSNA, K140I, RSNNS142-146PGSG), and antigenic site B (HL156-157KS, KFK158-160GST, NDQI189-192QEQT, A196V). "Native ELISA" analysis and escape mutant selection with two human monoclonal antibodies demonstrated that antibody E05 binds to antigenic site A and 1_C02 binds to site B. We find that most individuals, after vaccination in seasons 2006-07 and/or 2008-09, showed dominance of antigenic site B recognition over antigenic site A. A minority showed dominance of site A in 2006 but these were reduced in 2008 when the vaccine virus had a site A mutation. A better understanding of immunodominance may allow prediction of future antigenic drift and assist in vaccine strain selection. © 2012 Popova et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161970 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0041895 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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