Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16166
Title: Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
Authors: YANG YI
Keywords: Gene delivery, Baculovirus, virus surface modification, promoter engineering, central nervous system, glioma
Issue Date: 19-May-2007
Source: YANG YI (2007-05-19). Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system. ScholarBank@NUS Repository.
Abstract: Development of effective and safe gene delivery vectors is one of the most critical and challenging parts in gene therapy applications. In this study, we focused on the improvement of gene delivery efficiency of a newly emerged viral vector originated from insect viruses, namely baculovirus. Several important technical issues related to baculovirus vector production and purification processes were investigated. Through genetic modifications of Baculovirus, neuron-specific transgene expression was successfully achieved after local injection of the viral vector into the rat brain. A surface modification technology based on electrostatic interactions was developed to protect the envelope of the virus with a cationic polymer. The modified viral vectors were more resistant to serum inactivation and displayed an improved efficiency of transgene expression after systemic injection of the baculovirus vectors. With further characterization and modification, these viral gene delivery systems should have great potential in clinical central nervous system gene therapy.
URI: http://scholarbank.nus.edu.sg/handle/10635/16166
Appears in Collections:Ph.D Theses (Open)

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