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Title: Differential host immune responses in BALB/c and C57BL/6 mice to Burkholderia pseudomallei infection
Keywords: Burkholderia pseudomallei, melioidosis, inflammation, immunoglobulins, cytokines, IFN-gamma
Issue Date: 18-Jun-2007
Citation: KOO GHEE CHONG (2007-06-18). Differential host immune responses in BALB/c and C57BL/6 mice to Burkholderia pseudomallei infection. ScholarBank@NUS Repository.
Abstract: Burkholderia pseudomallei is the causative agent of melioidosis. Using two strains of mice showing differential susceptibility to B. pseudomallei infection, we found that BALB/c mice were highly susceptible to low-dose intranasal B. pseudomallei infection, whereas C57BL/6 mice were relatively resistant. A transient hyperproduction of IFN-gamma was found at 48h post-infection in the serum of BALB/c. It correlated with high bacterial loads in the infected organs, infiltration of leukocytes, and tissue pathology. C57BL/6 mice survived after first infection were found to be resistant to subsequent high dose challenge. Resistance of C57BL/6 mice to high dose infection correlates to high serum IgG2a, indicative of cell mediated immunity. BALB/c mice immunized with recombinant flagellin (r-FliC) induced high serum Th2-type Immunoglobulins (IgG1) which did not confer protection to infection. Na??ve BALB/c splenocytes produced higher IFN-gamma and other proinflammatory cytokines than na??ve C57BL/6 splenocytes. The IFN-gamma response was IL-12 and IL-18 dependent. Both NK cells and T cells were the major sources of IFN-gamma.
Appears in Collections:Ph.D Theses (Open)

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