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Title: Studies of shrimp white spot syndrome virus by protein array platform and proteomics approaches
Authors: CHEN JING
Keywords: protein array, wsv006, wsv254, proteomics, wsv010, wsv432
Issue Date: 21-Oct-2008
Citation: CHEN JING (2008-10-21). Studies of shrimp white spot syndrome virus by protein array platform and proteomics approaches. ScholarBank@NUS Repository.
Abstract: WSSV is the most serious pathogen in shrimp aquaculture recently. Gateway cloning technique was applied to construct clones of WSSV ORFs. The purified recombinant WSSV proteins were used for interaction screening of WSSV proteins with actin by protein array. The interaction of wsv006 with actin was confirmed by protein overlay assay and the interaction of wsv254 with actin was confirmed by co-immmunoprecipitation. The interactions with actin may help WSSV move inside the cell. Several viral proteins were found to interact with shrimp cytoskeleton and nuclear proteins respectively. More protein-protein interactions will be screened with protein array in future. Meanwhile, 45 viral proteins were identified by shotgun proteomics, 23 envelope proteins and 6 nucleocapsid proteins were identified by iTRAQ. Novel proteins wsv010 and wsv432 were shown to be envelope proteins. Furthermore, wsv010 was found to have interaction with VP24. This comprehensive study should facilitate the studies of WSSV infection mechanism.
Appears in Collections:Ph.D Theses (Open)

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