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Title: Prophylactic and therapeutic potential of synthetic peptides against enterovirus 71 (EV71)
Keywords: Enterovirus 71, HFMD, synthetic peptide, vaccine, neutralizing antibody, CD4+ T-cell
Issue Date: 13-Aug-2008
Citation: FOO GUANG WEI, DAMIAN (2008-08-13). Prophylactic and therapeutic potential of synthetic peptides against enterovirus 71 (EV71). ScholarBank@NUS Repository.
Abstract: Enterovirus 71 (EV71) causes Hand, foot and mouth disease (HFMD) and neither vaccine nor therapeutic treatment is currently available. In this study, antisera were raised in Balb/c mice against overlapping synthetic peptides spanning the VP1 capsid protein of EV71 strain 41. Two peptides, SP55 and SP70, elicited anti-EV71 neutralizing antibodies with SP70 eliciting a higher neutralizing antibody titer. Anti-SP70 antibodies were also able to confer good in vivo passive protection against homologous and heterologous EV71 strains in suckling Balb/c mice. This study also identified a major human IgG-specific immunodominant VP1 linear epitope by Pepscan analysis. To identify human CD4+ T-cell epitope, a virtual matrix-based prediction program (ProPred) was employed. Three predicted VP1 regions were tested in vitro for their abilities to induce CD4+ T-cell proliferation. Altogether, these can provide a better understanding in protective immunity and may help in the design of vaccines and diagnostic tools against EV71.
Appears in Collections:Ph.D Theses (Open)

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