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Title: Structural and functional studies on RNA modifying enzymes
Keywords: Ribosome, RNA modifying enzymes, Pseudouridine synthases, Methyltransferases, X-ray crystallography
Issue Date: 3-Aug-2008
Citation: SUNITA SUBRAMANIAN (2008-08-03). Structural and functional studies on RNA modifying enzymes. ScholarBank@NUS Repository.
Abstract: Of the post-transcriptional modifications in RNA, pseudouridylation and methylation are the most abundant. Pseudouridine synthases are enzymes that catalyze the isomerization of uridine to pseudouridine in structural RNA. Methyltransferases transfer methyl group/groups to a specific substrate (DNA, RNA, lipids, proteins and small molecules). This Ph.D. thesis comprises the elucidation of crystal structures of three enzymes from E.coli, namely RluF that modifies U2604 in 23S rRNA (Sunita et al., 2006, J Mol Biol, 359, 998-1009.), RsmC that methylates G1207 of 16S rRNA (Sunita et al., 2007, Nucleic Acids Resi, 35, 4264-4274) and RlmI that modifies 23S rRNA at nucleotide position C1962 (Sunita et al., 2008, J Mol Biol (in press)). The structural studies in each case were accompanied by a host of other biophysical, biochemical, functional and bioinformatics analyses. The results from the combination of experiments have not only thrown more light on the mechanism of substrate recognition, but have also revealed some fascinating details about the evolutionary pathway of these enzymes.
Appears in Collections:Ph.D Theses (Open)

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