Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16016
Title: Carbohydrate-centered pamam dendrimers for use in growing liver cells
Authors: JEREMY DANIEL LEASE
Keywords: dendrimer, PAMAM, hep3B, galactose, hydrogel, crosslinking
Issue Date: 23-Jul-2008
Source: JEREMY DANIEL LEASE (2008-07-23). Carbohydrate-centered pamam dendrimers for use in growing liver cells. ScholarBank@NUS Repository.
Abstract: Tissue engineering, specifically targeting the liver, is becoming increasingly important as donor organs continue falling shorter of medical needs and there is a growing demand for alternative tissue sources. Here, galactose-centered PAMAM dendrimers were synthesized for use as scaffold materials in the support of liver cell growth. Amine endgroups were first acquired on a galactose core molecule, followed by PAMAM dendrimer branching to higher generations. Dendrimers were surface modified with a galactose moiety to improve biocompatibility and enhance cell-scaffold interactions. Dendrimer particles were crosslinked into hydrogels using poly(ethylene glycol diglycidyl ether), poly(ethylene glycol diacrylate) and glutaraldehyde crosslinking agents. Increased swelling was observed for gels composed of higher dendrimer concentrations. Hep3B cell cultures exhibited maintained survivability and increased and extended cellular P450 production with a number of synthesized materials, signifying the potential of dendrimer constructed scaffolds towards application in cell culture support.
URI: http://scholarbank.nus.edu.sg/handle/10635/16016
Appears in Collections:Ph.D Theses (Open)

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