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Title: Anti-cancer effects of aloe-emodin: cellular and proteomics studies
Keywords: aloe-emodin proteomics anti-cancer ROS apoptosis peroxiredoxin
Issue Date: 22-Jul-2008
Source: LU GUODONG (2008-07-22). Anti-cancer effects of aloe-emodin: cellular and proteomics studies. ScholarBank@NUS Repository.
Abstract: Aloe-emodin (AE) is a major bioactive hydroxyanthraquinone in Chinese herbal medicine Rhubarb (Rheum palmatum). To better understand its anti-cancer mechanism, a systemic study integrating functional proteomics and conventional biochemical approaches was thus conducted. 2D-DIGE analysis revealed that AE affected various proteins in HepG2 cells, which are functionally associated with oxidative stress, cell cycle arrest, anti-metastasis. Biochemical validation then revealed several novel anti-cancer functions of AE. Firstly, AE induced ROS-dependent apoptosis through sustained ASK1/JNK activation. Secondly, AE inhibited DNA synthesis via induction of tumor suppressor p16. Thirdly, AE up-regulated nucleoside diphosphate kinase A (a metastasis suppressor), which may be account for AE-induced inhibition of cell migration/invasion. In summary, the present study, integrating the functional proteomics and biochemical approach, provides a comprehensive understanding of AE-induced anti-cancer effects. The proteomic approach offers an excellent technological platform for identification of the target molecules and the biochemical approach reveal the relevant pathways.
Appears in Collections:Ph.D Theses (Open)

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