Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15981
Title: The transcriptional induction and cellular localization study on two neuronal proteins: Neurogranin and GAP43
Authors: SONG YAN
Keywords: Neurogranin, GAP43, CaM, induction, localization, co-localization.
Issue Date: 4-Jun-2008
Source: SONG YAN (2008-06-04). The transcriptional induction and cellular localization study on two neuronal proteins: Neurogranin and GAP43. ScholarBank@NUS Repository.
Abstract: Two neuron-specific proteins, neurogranin (Ng), and neuromodulin (GAP43), were studied. Our first aim was to know the potentiality of various chemicals for their induction of Ng mRNA expression; our second one was to know the intracellular localization of GAP43 and its co-localization with calmodulin (CaM). Our results indicated that although some of the NO donors could enhance Ng promoter activity, they could not induce Ng mRNA expression in HEK293 and PC12 cells. Moreover, our findings revealed that Ng mRNA could be induced only by NGF and only in PC12 cells. For GAP43b s intracellular localization and co-localization with CaM, our results revealed DsRed-GAP43 and its mutants (S41D, S41G) were expressed mainly in the region near to cell membrane while EGFP-GAP43 and its mutants (S41D, S41G) were mostly distributed in cytoplasm. PMA treatment did not affect the localization of GAP43. For the co-transfection experiments, a particular region of co-localization of GAP43 and CaM was found to be close to the cell membrane.
URI: http://scholarbank.nus.edu.sg/handle/10635/15981
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
SongYans thesis.pdf2.45 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

170
checked on Dec 2, 2017

Download(s)

149
checked on Dec 2, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.