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https://scholarbank.nus.edu.sg/handle/10635/15949
DC Field | Value | |
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dc.title | Osmoregulation and excretory nitrogen metabolism in the euryhaline four-eyed sleeper, bostrychus sinensis, exposed to salinity and ammonia stresses | |
dc.contributor.author | PEH YEN XIAN, WENDY | |
dc.date.accessioned | 2010-04-08T10:59:14Z | |
dc.date.available | 2010-04-08T10:59:14Z | |
dc.date.issued | 2009-04-18 | |
dc.identifier.citation | PEH YEN XIAN, WENDY (2009-04-18). Osmoregulation and excretory nitrogen metabolism in the euryhaline four-eyed sleeper, bostrychus sinensis, exposed to salinity and ammonia stresses. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/15949 | |
dc.description.abstract | This study examines the effects of seawater and ammonia stress on nitrogen metabolism and osmoregulatory acclimation of euryhaline four-eyed sleeper, <i>Bostrychus sinensis</i>. <i>B. sinensis</i>, a facultative air-breather, exhibited hypoosmotic hypoionic osmoregulation in seawater. A novel finding here is that Na<sup>+</sup>/K<sup>+</sup>/Cl<sup>-</sup> cotransporter, and not Na<sup>+</sup>/K<sup>+</sup>-ATPase, was up-regulated in the gills of <i>B. sinensis</i> after long-term acclimation to seawater. On the other hand, Na<sup>+</sup>/K<sup>+</sup>-ATPase was up-regulated in the intestine. Exposure to ammonia in 5 parts per thousand water or seawater led to significant increases in glutamine contents and glutamine synthetase in various tissues of fish. In addition, exposure to 15 mmol l<sup>-1</sup> NH<sub>4</sub>Cl in seawater for 6 days resulted in a significant increase in the amination activity of glutamate dehydrogenase (GDH) from the intestine and a significant increase in the affinity of the GDH from the liver to NH<sub>4</sub><sup>+</sup>. Subsequently, two isoforms of GDH were identified and sequenced from <i>B. sinensis</i>. | |
dc.language.iso | en | |
dc.subject | ammonia, Bostrychus sinensis, nitrogen metabolism, glutamate dehydrogenase, gills, intestine | |
dc.type | Thesis | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.supervisor | IP YUEN KWONG | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
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PehYXW01.pdf | 171.28 kB | Adobe PDF | OPEN | None | View/Download | |
PehYXW02.pdf | 89.4 kB | Adobe PDF | OPEN | None | View/Download | |
PehYXW03.pdf | 2.61 MB | Adobe PDF | OPEN | None | View/Download | |
PehYXW04.pdf | 2.1 MB | Adobe PDF | OPEN | None | View/Download | |
PehYXW05.pdf | 3.55 MB | Adobe PDF | OPEN | None | View/Download | |
PehYXW06.pdf | 1.47 MB | Adobe PDF | OPEN | None | View/Download |
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