Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15949
Title: Osmoregulation and excretory nitrogen metabolism in the euryhaline four-eyed sleeper, bostrychus sinensis, exposed to salinity and ammonia stresses
Authors: PEH YEN XIAN, WENDY
Keywords: ammonia, Bostrychus sinensis, nitrogen metabolism, glutamate dehydrogenase, gills, intestine
Issue Date: 18-Apr-2009
Source: PEH YEN XIAN, WENDY (2009-04-18). Osmoregulation and excretory nitrogen metabolism in the euryhaline four-eyed sleeper, bostrychus sinensis, exposed to salinity and ammonia stresses. ScholarBank@NUS Repository.
Abstract: This study examines the effects of seawater and ammonia stress on nitrogen metabolism and osmoregulatory acclimation of euryhaline four-eyed sleeper, <i>Bostrychus sinensis</i>. <i>B. sinensis</i>, a facultative air-breather, exhibited hypoosmotic hypoionic osmoregulation in seawater. A novel finding here is that Na<sup>+</sup>/K<sup>+</sup>/Cl<sup>-</sup> cotransporter, and not Na<sup>+</sup>/K<sup>+</sup>-ATPase, was up-regulated in the gills of <i>B. sinensis</i> after long-term acclimation to seawater. On the other hand, Na<sup>+</sup>/K<sup>+</sup>-ATPase was up-regulated in the intestine. Exposure to ammonia in 5 parts per thousand water or seawater led to significant increases in glutamine contents and glutamine synthetase in various tissues of fish. In addition, exposure to 15 mmol l<sup>-1</sup> NH<sub>4</sub>Cl in seawater for 6 days resulted in a significant increase in the amination activity of glutamate dehydrogenase (GDH) from the intestine and a significant increase in the affinity of the GDH from the liver to NH<sub>4</sub><sup>+</sup>. Subsequently, two isoforms of GDH were identified and sequenced from <i>B. sinensis</i>.
URI: http://scholarbank.nus.edu.sg/handle/10635/15949
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