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Title: Effects of serial passaging on west nile virus (sarafend) in a mouse model
Keywords: West Nile, Mouse, Virus, passaging, mutation,
Issue Date: 18-Apr-2009
Citation: CHIANG CERN CHER, SAMUEL (2009-04-18). Effects of serial passaging on west nile virus (sarafend) in a mouse model. ScholarBank@NUS Repository.
Abstract: West Nile virus represents an emerging infectious disease and causes symptoms ranging from febrile illness to fatal encephalitis. WNV (Sarafend) having been passaged through cell culture, has lost its ability to infect and kill mice. This study seeks to revive the virusb murine infectability by repeatedly passaging it through mice brains and a mosquito cell line, mimicking normal infectious cycle. Passaged WNV was more persistent in adult BALB/c mice, causing severe pathogenesis while infected suckling mice had overall lower mean survival time. The passaged virus gave higher titres for longer periods on primary neural cultures. Sequencing found mutations in two hot spots in the C protein at amino acid residue 24 and 26. The region encompassing the first six amino acids for the NS2a gene was also found variable in the passaged viruses. These mutations are speculated to be important for virulence and adaptation of the virus to murine hosts.
Appears in Collections:Master's Theses (Open)

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