Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15836
Title: The NR4A orphan nuclear receptors are target genes of the novel drug C1 in cancer cells and potential mediators of drug induced apoptosis
Authors: KALA RAMASESHAN
Keywords: NR4A receptors, cancer, drug, apoptosis, VDAC1
Issue Date: 25-Apr-2009
Source: KALA RAMASESHAN (2009-04-25). The NR4A orphan nuclear receptors are target genes of the novel drug C1 in cancer cells and potential mediators of drug induced apoptosis. ScholarBank@NUS Repository.
Abstract: The present study was designed to decipher the mechanism of novel drug C1 mediated cell death. A microarray analysis was performed to study the effect of the C1 at various time points upon HL60 (a human promyelocytic leukemia cell line) cells. The analysis showed that a large number of transcripts are upregulated in the early time points including the orphan nuclear receptor NR4A3. This study has validated here by real time PCR the upregulation of NR4A3, and also NR4A1 which is a member of the same family of receptors. This study characterizes the role of NR4A1 and NR4A3 in C1 mediated apoptosis and has identified the functional relevance of the increase in the transcript level of NR4A1 and NR4A3. This thesis shows that in MCF-7 breast cancer cells, silencing NR4A receptors attenuates its reponse to low dose drug treatment by a mechanism that may involve the mitochondrial protein VDAC1
URI: http://scholarbank.nus.edu.sg/handle/10635/15836
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