Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15810
Title: Functional genomics in rice (Oryza sativa L) using Ac/Ds transposon tagging system and gene expression profiling
Authors: RAMAMOORTHY RENGASAMY
Keywords: Rice, Oryza sativa, Ac/Ds, Transposon tagging, WRKY, GRAM, RIP, Gene expression profiling
Issue Date: 12-May-2009
Source: RAMAMOORTHY RENGASAMY (2009-05-12). Functional genomics in rice (Oryza sativa L) using Ac/Ds transposon tagging system and gene expression profiling. ScholarBank@NUS Repository.
Abstract: A large pool of Ds transposants was generated using Ac/Ds transposon system and a semi-dwarf mutant was identified in the phenotype screen and in this mutant, the Ds had inserted into a cytochrome P450 gene (OsCYP96B4) causing the phenotype. Expression analysis of OsCYP96B4 revealed low and high expression levels in the vegetative and reproductive tissues, respectively. Revertant analysis, RNAi, and genetic complementation of the mutant confirmed that the phenotype was caused by the disruption of OsCYP96B4 gene. Subsequently, the over and ectopic expression of this gene in WT plants mimicked the mutant phenotype in a dosage dependent manner showing a negative correlation. In addition, in order to identify and characterize multi-stress responsive genes and develop elite rice lines with enhanced stress tolerance, we have profiled the expression of multi-gene families like GRAM, RIP and WRKY domain-encoding families by RT-PCR and qRT-PCRs. Our study revealed several independent genes that were responsive to multiple stresses.
URI: http://scholarbank.nus.edu.sg/handle/10635/15810
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