Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15804
Title: Endopolyploidy in dendrobium chao praya smile and anthurium andraeanum cv 'red hot'
Authors: KOH TENG SEAH
Keywords: Dendrobium, Orchid, Anthurium andraeanum, Flow cytometry, Endopolyploidy, Somaclonal variation
Issue Date: 28-Jul-2009
Source: KOH TENG SEAH (2009-07-28). Endopolyploidy in dendrobium chao praya smile and anthurium andraeanum cv 'red hot'. ScholarBank@NUS Repository.
Abstract: Endopolyploidy profiles of greenhouse-grown and aseptically-grown Dendrobium Chao Praya Smile and Anthurium andraeanum cv 'Red Hot' were investigated using flow cytometric analysis. For D. Chao Praya Smile, the occurrence of systemic endopolyploidy was detected in both greenhouse-grown plants and aseptically-grown seedlings. Endoreduplication was developmentally regulated in the protocorms, leaves and roots, but not in the flowers. Culture media containing BA resulted in a decrease in endoreduplication in the cells of protocorms. Conversely, the presence of 2,4-D or GA3 in the medium resulted in an increase in endopolyploidy. Addition of TIBA or PAC to the culture medium only inhibited endoreduplication in the cells of protocorms after 6 weeks of culture. Conversely, nuclei of greenhouse-grown plants and tissue-cultured plantlets of A. andraeanum cv 'Red Hot' were relatively stable with minimal ploidy variations. Addition of 2,4-D or BA to the medium had no effect on endopolyploidy in the callus tissues.
URI: http://scholarbank.nus.edu.sg/handle/10635/15804
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