Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15791
Title: Functional Lipidomics analysis of retrovirus envelopes
Authors: CHAN LER MIN, ROBIN BARRY
Keywords: retrovirus, lipidomics, phosphoinositides, glycosphingolipids, aminophospholipids
Issue Date: 8-Jun-2009
Source: CHAN LER MIN, ROBIN BARRY (2009-06-08). Functional Lipidomics analysis of retrovirus envelopes. ScholarBank@NUS Repository.
Abstract: To better understand how lipids contribute to the budding of retroviruses, we purified HIV and MLV (Murine Leukemia Virus) particles and characterized their lipid inventory. We discovered that retrovirus particles are generally enriched in so called raft lipids such as cholesterol, ceramide and glycosphingolipid GM3 and also the signaling lipid phosphatidylinositol bisphosphate PIP2. Based on the initial lipidomics analysis, we chose three groups of retrovirus envelope lipids for further characterization. First, we mapped the source of PIP2 enrichment to the MA domain of retrovirus Gag structural protein and showed that cellular PI(4,5)P2 is needed for retrovirus budding. Second, we modulated cellular GM3 levels and showed that it is required for the production of viable MLV particles. Lastly, we showed that the fusogenic lipid plasmalogen phosphatidylethanolamine is highly enriched in the outer shell of MLV particles.Therefore, our data shows that specific lipids are functionally enriched in retrovirus envelopes through virus-host interactions.
URI: http://scholarbank.nus.edu.sg/handle/10635/15791
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