Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15786
Title: The ancient origin of NF-kB/IkB and thioredoxin and their roles in immune response
Authors: WANG XIAOWEI
Keywords: Horseshoe crab, IkB, immune response, NF-kB, thioredoxin, signal transduction
Issue Date: 26-Jan-2007
Source: WANG XIAOWEI (2007-01-26). The ancient origin of NF-kB/IkB and thioredoxin and their roles in immune response. ScholarBank@NUS Repository.
Abstract: This thesis reports the discovery and characterization of a primitive but functional NF-kB/IkB cascade in a a??living fossila??, the horseshoe crab, demonstrating the earliest origin of this signaling cascade. The horseshoe crab NF-kB/IkB homologues share numerous signature motifs with their vertebrate orthologues. In vitro and in vivo functional studies showed that the ancient NF-kB pathway plays a fundamental role in regulating the expression of immune defense molecules and is functionally comparable to that of the Drosophila and mammals, suggesting that the roles of NF-kB and IkB have co-evolved through evolution. Furthermore, we discovered a novel 16 kDa thioredoxin from the horseshoe crab, designated Cr-TRX1. Although the Cr-TRX1 is larger than the classical 12 kDa counterpart and contains an atypical catalytic motif, it possesses the classical thioredoxin activity. Cr-TRX1 could activate the NF-kB activity by enhancing its DNA-binding activity, suggesting possible roles of the Cr-TRX1 in modulating NF-kB signaling pathway.
URI: http://scholarbank.nus.edu.sg/handle/10635/15786
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
WangXW.pdf2.96 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

317
checked on Dec 18, 2017

Download(s)

267
checked on Dec 18, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.