Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15768
Title: Long wave ultra sound may enhance bone remodelling by altering the opg/rankl ratio in human osteoblast like cells
Authors: MADDI ABHIRAM
Keywords: Osteoblasts, OPG, RANKL, Long-wave Ultrasound
Issue Date: 31-Dec-2006
Source: MADDI ABHIRAM (2006-12-31). Long wave ultra sound may enhance bone remodelling by altering the opg/rankl ratio in human osteoblast like cells. ScholarBank@NUS Repository.
Abstract: Human osteoblast-like cells (MG63) were treated with long wave (45 kHz, 30 mW/cm2) continuous ultrasound (US) for 5 min and incubated for various time periods. The reverse transcriptase polymerase chain reaction (RT-PCR) technique was used for observing the genetic expression and real-time PCR for quantitative analysis of receptor activator of NF-I?B ligand (RANKL) and osteoprotegerin (OPG) along with alkaline phosphatase (ALP), an early bone marker, and osteocalcin (OCN) a late marker. ELISA was performed to estimate the amount of the cytokine released into the culture media. The osteoblasts responded to US by significantly upregulating both the OPG mRNA and protein levels. There was no RANKL mRNA expression observed in both the US and control groups and the protein levels were also very low in both groups. There was also no TNF-I? expression and it's protein levels were insignificant. ALP and OCN mRNA were significantly upregulated in the US group.
URI: http://scholarbank.nus.edu.sg/handle/10635/15768
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