Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15694
Title: Interaction between legionella pneumophila and biofilm forming organism pseudomonas aeruginosa
Authors: WON CHOONG YUN
Keywords: Pseudomonas aeruginosa biofilm, porosity, Legionella pneumophila, adhesion, persistence, 2,2-dibromo-3-nitrilopropionamide (DBNPA)
Issue Date: 5-Mar-2007
Source: WON CHOONG YUN (2007-03-05). Interaction between legionella pneumophila and biofilm forming organism pseudomonas aeruginosa. ScholarBank@NUS Repository.
Abstract: A model Pseudomonas aeruginosa PAO1-CFP biofilm, with distinct developmental stages, was established in CDC Biofilm Reactor continuous flow system. Adhesion of Legionella pneumophila to the biofilms was influenced by the structure and porosity, but not developmental stage, of the latter. With or without unbalanced advective transport of legionellae to the surface of biofilm that commenced upon biofilm maturation, legionellae was least likely to desorb from bottom 60% of the biofilms, especially at bottom 20%. Biofilms with associated legionellae were treated with biocide, NALCO 7320, which contained 2,2-dibromo-3-nitrilopropionamide (DBNPA) as the active ingredient. Legionellae persistence in biocide-treated biofilms was independent on biofilm developmental stage and loss of biomass, but there existed regions of the biofilms in which legionellae best persist. Since extracellular polysaccharide (EPS) is a major component in biofilm matrix, we hypothesize that EPS plays an important role in legionellae persistence in biocide-treated biofilms.
URI: http://scholarbank.nus.edu.sg/handle/10635/15694
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Won Choong Yun.pdf3.84 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

322
checked on Dec 11, 2017

Download(s)

367
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.