Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15629
Title: Diamond-based biosensor
Authors: GU HUIRU
Keywords: Boron-doped diamond, Biosensor, Impedance, SPR
Issue Date: 7-Dec-2006
Source: GU HUIRU (2006-12-07). Diamond-based biosensor. ScholarBank@NUS Repository.
Abstract: This thesis examines the surface modification of boron-doped diamond (BDD) with a view in developing it as the signal transduction platform for biomolecules. Surface modification methods based on the electro-polymerization of polyaniline-acrylate on BDD, as well as the photochemical coupling of organic molecules on BDD, were investigated. Bio-recognition events based on the hybridization of complimentary and mismatched DNA sequences were studied using electrochemical methods such as cyclic voltammetry and electrochemical impedance spectroscopy. By studying the frequency dependence of the impedimetric response, the physical origins of the impedimetric responses were shown to be related either to changes in the semiconductor space charge changes, or to the changes in surface adlayer resistance, depending on the surface modification methods which immobilize the probe DNA on the BDD surfaces. Finally, another polymer system based on poly-4-nitro-1,2 phenylenediamine (P4NoPD) were investigated as a possible matrix for immobilizing biomolecules. It was shown that Surface Plasmon Resonance can be the signal transducer for the different redox states of P4NoPD. Using a model biomolecular system, involving streptavidin, biotinylated DNA and its complementary target DNA, it was found that the presence of nitro groups in P4NoPD allows the biorecognition events to be modulated by voltages.
URI: http://scholarbank.nus.edu.sg/handle/10635/15629
Appears in Collections:Ph.D Theses (Open)

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2-Abstract.pdf8.44 kBAdobe PDF

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3-Table of Content.pdf22.07 kBAdobe PDF

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5-List of figures.pdf187.37 kBAdobe PDF

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7-Summary.pdf31.48 kBAdobe PDF

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9-Appendix.pdf6.95 kBAdobe PDF

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