Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15594
Title: Pharmacological and structural characterization of novel taxa-specific neurotoxins from Boiga venoms
Authors: PAWLAK JOANNA
Keywords: three-finger toxins, glutaminyl cyclase, venom, colubrid snakes, neurotoxicity, nicotinic acetylocholine receptor
Issue Date: 28-Nov-2006
Source: PAWLAK JOANNA (2006-11-28). Pharmacological and structural characterization of novel taxa-specific neurotoxins from Boiga venoms. ScholarBank@NUS Repository.
Abstract: Snake venoms are a complex mixture of bioactive peptides and proteins that encompass an arsenal of three-finger neurotoxins. Two novel three-finger toxins, namely denmotoxin (monomer) and irditoxin (covalently-linked heterodimer) were isolated, purified and sequenced from rear-fanged colubrid snakes Boiga dendrophila and Boiga irregularis respectively. Detailed pharmacological characterization of these venom components on different vertebrate species showed high specie-specific neurotoxicity and high resolution crystal structures revealed many structurally unique features. Further, chemical synthesis of 77 amino acid residues long denmotoxin followed by the development of a refolding protocol helped to overcome its low natural abundance, while sequencing of denmotoxin gene revealed a unique organization with an additional forth exon. Present study provides the first detailed account of the structure and function of colubrid taxa-specific three-finger neurotoxins, which can contribute to greater understanding of specie-specific ligand recognition properties and function of nicotinic acetylcholine receptors.
URI: http://scholarbank.nus.edu.sg/handle/10635/15594
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