Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15509
Title: Investigate the Function of Kinectin Isoforms in Endoplasmic Reticulum Dynamics
Authors: ZHU YAJUAN
Keywords: Kinectin, Kinesin, ER, Migration, Mitosis, Cellular-imaging
Issue Date: 16-Sep-2006
Source: ZHU YAJUAN (2006-09-16). Investigate the Function of Kinectin Isoforms in Endoplasmic Reticulum Dynamics. ScholarBank@NUS Repository.
Abstract: The function of kinectin isoforms during ER dynamcis was addressed in this project using multiple molecular and cellular imaging techniques. The subcellular localization of kinectin isoforms was studied by injecting EGFP-tagged chimera into a DsRed-ER HeLa line. Confocal microscopy imaging revealed significant co-localization of 160 kDa kinectin with ER and diffused distribution pattern for 120 kDa isoforms. In addition, 160 kDa isoforms with Int4 co-accumulated with ER in the leading edges of migrating cells. RNAi and overexpression studies further confirmed that 160 kDa kinectin with Int4 anchored ER to kinesin which then contributed to cell migration by transporting ER to the leading edge along microtubles. The importance of kinectin in ER dynamics during cell division was also investigated using morpholinos which specifically knocked down Int4. However, neither the overall ER dynamics nor the association with spindle was affected, indicating ER dynamics during mitosis might be independent of kinesin-kinectin interaction.
URI: http://scholarbank.nus.edu.sg/handle/10635/15509
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
E-Theis submission.pdf3.64 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

177
checked on Dec 11, 2017

Download(s)

248
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.