Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15462
Title: Role of glycosphingolipids in formation and function of lipid rafts
Authors: MIAO LV
Keywords: Lipid rafts, GSLs, DRMs, NB-DNJ, Endocytosis, CTxB
Issue Date: 7-Jun-2006
Source: MIAO LV (2006-06-07). Role of glycosphingolipids in formation and function of lipid rafts. ScholarBank@NUS Repository.
Abstract: Lipid rafts are ordered membrane domains which are enriched in cholesterol and glycosphingolipids (GSLs). They may play important roles in various cellular functions including membrane trafficking and signaling. Lipid rafts could be extracted by treatment with cold Triton X-100 as the detergent resistant membranes (DRMs) from the cell membranes. It is believed that the interactions among headgroups of GSLs are essential for the formation of functional rafts. To investigate the roles of GSLs in rafts, a GSL biosynthesis inhibitor, N-Butyldeoxynojirimycin (NB-DNJ), was employed to deplete the cellular GSLs in human fibroblast cells. The results obtained in this study show that the level of cellular GM3 and cell surface GM1 were significantly decreased following treatment with NB-DNJ. As a result, raft formation in the plasma membranes was decreased by NB-DNJ in a dose-dependent manner, so was the raft-dependent endocytosis of cholera toxin subunit B (CTxB), suggesting that inhibition of de nova GSL biosynthesis does affect raft formation and its function in human fibroblast cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/15462
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