Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15452
Title: Generation of mouse graves' ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real-time PCR
Authors: GOH SUI SIN
Keywords: Graves Ophthalmopathy, Animal model, Genetic immunization, TSH Receptor, Cytokine Profile, Real-Time PCR
Issue Date: 7-Jun-2006
Source: GOH SUI SIN (2006-06-07). Generation of mouse graves' ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real-time PCR. ScholarBank@NUS Repository.
Abstract: Gravesa?? ophthalmopathy (GO) is a potentially disfiguring, sight-threatening and frequent complication of Gravesa?? disease. Lymphocytic infiltration of muscular and connective tissues of the retroorbital space is a histological hallmark of GO. Study of inflammatory processes and cytokine profiling in human tissue samples were limited by sample, genetic and technique heterogeneity. It is the aim of this study, to investigate the spectrum of T-lymphocyte cytokines expressed in tissues of genetically immunized inbred Balb/c and outbred Swiss mice by means of Real-Time PCR. These mice were injected with plasmid encoding the TSHR gene. The cytokine profile evaluation in the orbital tissues showed down regulation of IL5 in Balb/c and I?IFN, IL4 and IL5 in Swiss outbred mice, thus implying a relatively quiescent immunological environment in this tissue compartment. Therefore, dominance of either Th1 or Th2 response cannot be determined with confidence. The results showed genetic immunization worked better in Swiss outbred than Balb/c.
URI: http://scholarbank.nus.edu.sg/handle/10635/15452
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