Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15401
Title: Monolithic and perfusive packings: A study of surface chemistry and mass transfer in protein separation
Authors: NE LIN
Keywords: Monolithic columns; perfusion columns; hydrodynamics, surface energetic heterogeneity; mass transfer, axial dispersion
Issue Date: 26-May-2006
Source: NE LIN (2006-05-26). Monolithic and perfusive packings: A study of surface chemistry and mass transfer in protein separation. ScholarBank@NUS Repository.
Abstract: Both the monolithic and perfusive chromatographic media have bimodal pore size distribution which contributes to higher column throughput and higher column efficiencies compared to conventional particulate stationary phases. In this research, a systematic comparative study on these two types of column media was performed to elucidate the similarities and differences of them and also to further understand the hydrodynamic, surface chemistry and mass transfer behaviors of these media. Two kinds of reversed-phase perfusive media (POROS R1/H and POROS R2/H) and two monolithic columns (a polymer-based Monolithic RP-pro and a silica based Monolithic RP-18e column) were used for comparison. A significant difference in pore size distribution, pore geometry and thermodynamic property was revealed among these media; Monolithic RP18e had the most homogeneous while Monolithic RP-pro the most heterogeneous pore size distributions. The effects of pore size, porosity, solute molecular sizes and flow velocity on axial dispersion and column efficiency were also examined.
URI: http://scholarbank.nus.edu.sg/handle/10635/15401
Appears in Collections:Master's Theses (Open)

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