Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15280
Title: Molecular Analysis of Oxysterol Binding Proteins in Yeast
Authors: WANG PENG HUA
Keywords: AAA ATPase, oxysterol binding protein, sterol homeostasis, membrane association and lipid transport.
Issue Date: 30-Mar-2006
Source: WANG PENG HUA (2006-03-30). Molecular Analysis of Oxysterol Binding Proteins in Yeast. ScholarBank@NUS Repository.
Abstract: Oxysterol binding protein (OSBP) and its homologues constitute a large family of conserved proteins, which are implicated in lipid metabolism and vesicular transport. Here, we characterized the yeast OSBP homologues including Osh6p in great details and demonstrated that Osh6p was localized to both cytosol and membranes. Deletion or overexpression of OSH6 resulted in changes in sterol homeostasis but appeared to have no effect on endocytic or biosynthetic transport to the vacuole. Furthermore we showed that Vps4p-an AAA ATPase interacted with Osh6p and Osh7p, and regulated their membrane association. Interestingly, we found another AAA ATPase-Afg2p regulated the membrane association of Osh1p, and both Vps4p and Afg2p regulated sterol metabolism. These data suggest that a common mechanism exist for certain AAA proteins to regulate the membrane association of Osh and that these two families function together to control subcellular sterol/membrane transport.
URI: http://scholarbank.nus.edu.sg/handle/10635/15280
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