Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15122
Title: Global analysis of protein expression in focal cerebral ischemia in rats
Authors: CHEN AIQING
Keywords: cerebral ischemia, protein expression, dihydropyrimidinase-related protein 2, spectrin alpha II chain, HSC70-ps1, tropomodulin 2
Issue Date: 5-Mar-2006
Source: CHEN AIQING (2006-03-05). Global analysis of protein expression in focal cerebral ischemia in rats. ScholarBank@NUS Repository.
Abstract: Protein expression was investigated in the rat cortex 24 h after occlusion of the middle cerebral artery (MCAO) via two-dimensional electrophoresis. At pH 4-7, 52 protein spots were changed with 2-3-fold between sham and MCAO samples, and 13 spots were changed over 3-fold; while at pH 6-11, only 4 spots showed more than 2-fold changes. The 13 spots with 3-fold changes at pH 4-7 and the 4 spots at pH 6-11 were chosen for spot identification, and 12 of them were identified as 9 different proteins: dihydropyrimidinase-related protein 2 (DRP-2, accounting for 3 spots), spectrin alpha II chain (accounting for 2 spots), tropomodulin 2, beta-actin, N-ethylmaleimide sensitive factor (NSF), heat hock protein cognate 70-pseudogene 1, and glyceraldehydes 3-phosphate dehydrogenase are increased after MCAO; mitochondrial H+-ATP synthase alpha subunit and heterogeneous nuclear ribonucleoprotein A2/B1 are reduced. As DRP-2, spectrin alpha II chain, tropomodulin 2 and NSF are involved in axonal and neurite growth as well as synaptic plasticity and maturation, the upregulation of these proteins may indicate active neuroregeneration after cerebral ischemia.
URI: http://scholarbank.nus.edu.sg/handle/10635/15122
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
COVER FOR ChenAQ 2005.pdf9.07 kBAdobe PDF

OPEN

NoneView/Download
SIDE COVER FOR ChenAQ 2005.pdf7.73 kBAdobe PDF

OPEN

NoneView/Download
ChenAQ-01Chap.pdf99.06 kBAdobe PDF

OPEN

NoneView/Download
ChenAQ-02Chap.pdf2.63 MBAdobe PDF

OPEN

NoneView/Download
ChenAQ-03Chap.pdf227.97 kBAdobe PDF

OPEN

NoneView/Download

Page view(s)

227
checked on Dec 2, 2017

Download(s)

578
checked on Dec 2, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.