Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15059
Title: The utilization of biodegradable materials for the delivery of bioactive molecules that promote CNS regeneration
Authors: TAN YEN MEI, ELAINE
Keywords: Drug delivery, axonal regeneration, C3 transferase, PLGA, microspheres, release profile
Issue Date: 6-Feb-2006
Source: TAN YEN MEI, ELAINE (2006-02-06). The utilization of biodegradable materials for the delivery of bioactive molecules that promote CNS regeneration. ScholarBank@NUS Repository.
Abstract: Growth inhibitory proteins limit axonal regeneration in the injured spinal cord and a common target of these inhibitors is the Rho family of small GTPases. Activation of RhoA leads to growth cone collapse and neurite retraction. An enzyme from Clostridium botulinum, C3 transferase (C3), blocks RhoA function by ADP ribosylation of the effector domain, and this promotes regeneration of injured neurons. The aim of this work was to develop C3-loaded microspheres for implantation in the injured spinal cord. The approach of blending capped (hydrocarbon end-groups) and uncapped (free acid end-groups) poly(D,L-lactide-co-glycolide) (PLGA) to achieve continuous release of C3 was investigated. The microspheres were prepared by w/o/w emulsification and the in vitro release and degradation profile were characterised. Microspheres prepared from capped/uncapped PLGA with a blending ratio of 30/70 yielded continuous, average daily release of 3.0 ng/day/mg of microspheres of C3 in one month. This study demonstrates the feasibility of using PLGA microspheres for sustained delivery of C3.
URI: http://scholarbank.nus.edu.sg/handle/10635/15059
Appears in Collections:Master's Theses (Open)

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