Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15015
Title: Study of galactosylated collagen as ECM in 3-D hepatocyte culture in vitro
Authors: WU YINGNAN
Keywords: Galactosylated collagen; Microcapsule; Hepatocyte; Liver tissue engineering; In situ forming gel; ECM
Issue Date: 10-Nov-2005
Source: WU YINGNAN (2005-11-10). Study of galactosylated collagen as ECM in 3-D hepatocyte culture in vitro. ScholarBank@NUS Repository.
Abstract: The galactose group has been conjugated to the amino or carboxyl group on collagen molecules. Galactosylated was used as ECM for hepatocyte culture in two 3-D culture systems, which are microcapsules and porous PHEMA hydrogel scaffold.In microcapsule system, the character of mass transfer ans some physical characters have benn identified. The optimized cell functions of hepatocytes in microcapsules have been investigated, which were basing on different modification degree galactosylated collagen, different mixed ratio of methylated collagen and galactosylated collagen, and cell densities which were entrapped by microcapules.In Porous PHEMA scaffold and in situ forming galactosylated collagen system, the in situ forming modified collagen was prepared by interaction between galactosylated collagen and APBS. Hepatocytes were entrapped by in situ gelation galactosylated collagen in porous PHEMA scaffold. The material characters of PHEMA have been tested. The formation procedure of in situ felling collagen has been investigated. The cell functions of hepatocytes in this system have also been evaluated. Above all, galactosylated collagen can enhance cell functions of hepatocytes in 3-D culture system. The galactosylated collagen microcapsule system can be used as cell delivery device potentially. Otherwise, in situ forming galactosylated collagen system can be used as cell implantable system potentially, too.
URI: http://scholarbank.nus.edu.sg/handle/10635/15015
Appears in Collections:Master's Theses (Open)

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