Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14958
Title: Enzymatic kinetic resolution of racemic flurbiprofen
Authors: ZHANG HUAYUN
Keywords: Enzymatic kinetic resolution, Racemic flurbiprofen, Immobilized lipase, Activity, Enantioselectivity, Stability.
Issue Date: 29-Oct-2005
Source: ZHANG HUAYUN (2005-10-29). Enzymatic kinetic resolution of racemic flurbiprofen. ScholarBank@NUS Repository.
Abstract: In the thesis work, the enzymatic kinetic resolution of racemic flurbiprofen was studied via the lipase (NovozymA?435)-catalyzed enantioselective esterification. A practical procedure has been first developed to optimize the resolution, with a focus on solvent and alcohol types, inhibition of alcohol substrates and the nature of the reversible reaction. NovozymA?435 was proved to be a good-performance biocatalyst for the reaction with R-stereopreference in the solvent of cyclohexane. Hydrophobicity of organic solvents was found to play an important role in affecting the activity and enantioselectivity of the lipase. In addition, the kinetic study shows that the reaction matches Bi Bi Ping Pong mechanism with dead-end inhibition and R-enantiomer presents lower activation energy and higher maximum reaction rate than S-enantiomer, which implies the R-stereopreference of the lipase and makes the resolution of flurbiprofen via enzymatic reaction feasible. Besides, the stability study reveals the excellent chemical and thermal stability of the lipase in alkyl organic solvents.
URI: http://scholarbank.nus.edu.sg/handle/10635/14958
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
4_summary.pdf38.55 kBAdobe PDF

OPEN

NoneView/Download
5_list of symbol_figure_table.pdf74.46 kBAdobe PDF

OPEN

NoneView/Download
chapter 1.pdf50.01 kBAdobe PDF

OPEN

NoneView/Download
chapter 2.pdf94.65 kBAdobe PDF

OPEN

NoneView/Download
chapter 3.pdf191.03 kBAdobe PDF

OPEN

NoneView/Download
chapter 4.pdf204.19 kBAdobe PDF

OPEN

NoneView/Download
chapter 5.pdf235.21 kBAdobe PDF

OPEN

NoneView/Download
chapter 6.pdf111.7 kBAdobe PDF

OPEN

NoneView/Download
chapter 7.pdf49.91 kBAdobe PDF

OPEN

NoneView/Download
References.pdf37.28 kBAdobe PDF

OPEN

NoneView/Download

Page view(s)

286
checked on Dec 11, 2017

Download(s)

2,079
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.