Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14848
Title: Nanotechnology to understand virus-cell interactions
Authors: LEE WEI MING, JASON
Keywords: AFM, Virus, Force Interactions, West Nile virus envelope protein, Integrin αVβ3
Issue Date: 29-May-2005
Source: LEE WEI MING, JASON (2005-05-29). Nanotechnology to understand virus-cell interactions. ScholarBank@NUS Repository.
Abstract: The Atomic Force Microscope (AFM) was applied here in an extensive study in its applications to virology. The imaging aspect of the microscope was used to study virus-induced cell morphological changes, as well as to capture the minutiae of the virus-infection process, such as egression processes at very high resolution. Three virus models were used in this study; West Nile virus, dengue virus and Severe Acute Respiratory Syndrome coronavirus. Imaging was conducted in fixed virus-infected cell samples in both dry and liquid conditions. Living uninfected cells in a liquid environment were also observed. The metrological aspect of the AFM was exploited to quantitatively measure the interacting forces between the West Nile virus envelope protein domain III and its corresponding cellular receptor integrin I?VI?3. This would serve as a future drug-discovery platform, in which drugs that disrupt the virus binding process could be tested.
URI: http://scholarbank.nus.edu.sg/handle/10635/14848
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Literature Review.pdf992.58 kBAdobe PDF

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M&M.pdf569.68 kBAdobe PDF

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Results 1.pdf6.79 MBAdobe PDF

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Results 3.pdf1.97 MBAdobe PDF

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Discussion.pdf205.56 kBAdobe PDF

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References.pdf152.36 kBAdobe PDF

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Appendix.pdf211.92 kBAdobe PDF

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