Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/14829
Title: | A study into the different mechanisms of anti-cancer effect of docosahexanoic acid in colon cancer cells | Authors: | MANAV | Keywords: | DHA,colon, JNK,ROS,PPARgamma and COX-2 | Issue Date: | 26-May-2005 | Citation: | MANAV (2005-05-26). A study into the different mechanisms of anti-cancer effect of docosahexanoic acid in colon cancer cells. ScholarBank@NUS Repository. | Abstract: | Colon cancer is a leading cause of cancer mortality in the developed world. Epidemiological evidence suggests that n-3 polyunsaturated fatty acids (PUFAs) may protect against colon cancer. In this study we show that docosahexanoic acid (DHA)(22:6, n-3), induced caspase-dependent apoptosis in HT-29 colon cancer cells. DHA activated JNK, ERK and p38 MAPKs. Inhibition of JNK either by SP600125 or by overexpression of the dominant-nagative mutants significantly repressed DHA-induced apoptosis. DHA also induced oxidative stress but JNK activity and apoptosis were independent of the ROS generation. We further show that DHA activated PPARgamma and inhibited TNFalpha-induced COX-2 expression. Repressing the PPARgamma protein expression de creased the inhibitory effect of DHA on COX-2 expression. However, there were no significant changes in cell death indicating that DHA induced apoptosis through PPARgamma-independent pathway. In conclusion, this study provides an insight in to the different pathways involved anti-cancinogenic role of DHA in colon cancer. | URI: | http://scholarbank.nus.edu.sg/handle/10635/14829 |
Appears in Collections: | Master's Theses (Open) |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
Manav-MScThesis COFM-NUS.pdf | 897.05 kB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.