Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14799
Title: The hepatitis B core promoter: Control of viral life cycle events
Authors: ONG SHAIR LING, CARMEN
Keywords: HBV, core promoter, viral replication
Issue Date: 25-May-2005
Source: ONG SHAIR LING, CARMEN (2005-05-25). The hepatitis B core promoter: Control of viral life cycle events. ScholarBank@NUS Repository.
Abstract: The HBV core promoter transcribes both the pre-core and the pre-genomic mRNAfrom which translation of the HBeAg and HBcAg occurs respectively..This study aimed to investigate the importance of the CP in viral replication. Pairedsamples, pre- and post HBeAg seroconversion, were selected from 14 chronic HBVpatients who had a significant decrease in serum viral load after HBeAgseroconversion implying a drop in viral replication. The CP acitivty from each samplewas quantitated via a luciferase reporter gene. 5a?? RACE was also carried out todistinguish between pre-core and pre-genomic mRNA transcription. Sequenceanalysis showed the presence of previously reported and novel CP mutations whichcaused a change in CP activity. However, the correlation between CP activity andviral replication was not significant. Hence, we conclude that the regulation of HBVreplication is a complex process in which the CP plays a necessary, but not sufficient,role.
URI: http://scholarbank.nus.edu.sg/handle/10635/14799
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
01-Title Page.pdf6.61 kBAdobe PDF

OPEN

NoneView/Download
02-Table of Contents.pdf47.4 kBAdobe PDF

OPEN

NoneView/Download
03-Introduction.pdf218.55 kBAdobe PDF

OPEN

NoneView/Download
04-Methods.pdf437.28 kBAdobe PDF

OPEN

NoneView/Download
05-Results.pdf290.44 kBAdobe PDF

OPEN

NoneView/Download
06-Discussion.pdf63.64 kBAdobe PDF

OPEN

NoneView/Download
07-References.pdf49.91 kBAdobe PDF

OPEN

NoneView/Download
08-APPENDIX.pdf15.99 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

211
checked on Jan 19, 2018

Download(s)

1,051
checked on Jan 19, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.