Please use this identifier to cite or link to this item: https://doi.org/10.1007/s12640-016-9664-y
Title: Sirtuin 5 is Anti-apoptotic and Anti-oxidative in Cultured SH-EP Neuroblastoma Cells
Authors: Liang F.
Wang X.
Ow S.H.
Chen W.
Ong W.C. 
Keywords: Apoptosis
Mitochondria
Reactive oxygen species (ROS)
SH-EP neuroblastoma cell line
Sirtuin 5 (sirt5)
Staurosporine
Issue Date: 1-Jan-2017
Publisher: Springer New York LLC
Citation: Liang F., Wang X., Ow S.H., Chen W., Ong W.C. (2017-01-01). Sirtuin 5 is Anti-apoptotic and Anti-oxidative in Cultured SH-EP Neuroblastoma Cells. Neurotoxicity Research 31 (1) : 63-76. ScholarBank@NUS Repository. https://doi.org/10.1007/s12640-016-9664-y
Abstract: As a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, demalonylase, and desuccinylase, sirtuin 5 (SIRT5) in host cells has been reportedly observed in the mitochondria, in the cytosol/cytoplasm or in the nucleus. Various functional roles of SIRT5 have also been described in cellular metabolism, energy production, detoxification, oxidative stress, and apoptosis, but some of the reported results are seemingly inconsistent or even contradictory to one another. Using immunocytochemistry, molecular biology, gene transfection, and flow cytometry, we investigated the expression, subcellular distribution, and possible functional roles of SIRT5 in regulating apoptosis and oxidative stress of cultured SH-EP neuroblastoma cells. Both endogenous and transfected exogenous SIRT5 were observed in mitochondria of host SH-EP cells. Overexpression of SIRT5 markedly protected SH-EP cells from apoptosis induced by staurosporine or by incubation in Hank�s balanced salt solution. SIRT5 also lowered the level of oxidative stress and countered the toxicity of hydrogen peroxide to SH-EP cells. It was suggested that the anti-apoptotic role of SIRT5 was mediated, at least in part, by its anti-oxidative effect in SH-EP neuroblastoma cells although the involved molecular mechanisms remain to be elucidated in details. � 2016, Springer Science+Business Media New York.
Source Title: Neurotoxicity Research
URI: http://scholarbank.nus.edu.sg/handle/10635/146695
ISSN: 10298428
DOI: 10.1007/s12640-016-9664-y
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