Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14641
Title: Functional & structural studies of Stonustoxin (SNTX), a lethal factor from Stonefish (Synanceja Horrida) venom
Authors: LIEW HUEI CHUN
Keywords: Stonustoxin (SNTX), Hydrogen Sulfide (H2S), Nitric Oxide (NO), B30.2 domain, Chaperonin, Inclusion Bodies
Issue Date: 5-May-2005
Source: LIEW HUEI CHUN (2005-05-05). Functional & structural studies of Stonustoxin (SNTX), a lethal factor from Stonefish (Synanceja Horrida) venom. ScholarBank@NUS Repository.
Abstract: Stonustoxin (SNTX) is a 148kDa, dimeric, multifunctional protein isolated from stonefish Synanceja horrida. SNTX (10-640ng/ml) progressively causes vasorelaxation to endothelium-intact, PE-precontracted rat thoracic aortic rings. The vasorelaxation was inhibited by L-NAME, PAG and BCA. Use of L-NAME with PAG or BCA showed that H2S works synergistically with NO to bring about SNTX-induced vasorelaxation. This is the first report on the involvement of H2S working together with NO to mediate a toxina??s biological effect. Interestingly, an unexpected transient increase in tone of resting rat thoracic aortic rings was observed with L-cysteine. SNTX possesses a novel B30.2 domain in each subunit. Expression and purification of Glutathione-S-transferase-B30.2 fusion proteins from E. coli BL21 resulted in co-expression of chaperonin GroEL and aggregation of fusion proteins into inclusion bodies. The proteins were not active in vasorelaxation and further studies need to be conducted.
URI: http://scholarbank.nus.edu.sg/handle/10635/14641
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