Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14638
Title: Cometabolic transformation of 2- and 4-chlorophenol in the presence of phenol by Pseudomonas putida
Authors: WU TINGTING
Keywords: cometabolic transformation;chlorophenol; inhibition; toxicity; model; ternary substrate system
Issue Date: 21-Apr-2005
Source: WU TINGTING (2005-04-21). Cometabolic transformation of 2- and 4-chlorophenol in the presence of phenol by Pseudomonas putida. ScholarBank@NUS Repository.
Abstract: Biotransformation of 2-chlorophenol and 4-chlorophenol in the presence of phenol by Pseudomonas putida (ATCC 49451) was investigated in the laboratory. P. putida was unable to utilize 2-cp and 4-cp as the sole carbon and energy source. In the presence of phenol, 2-cp and 4-cp could be transformed through cometabolic pathway. It has been found that cell growth and phenol degradation were strongly inhibited by the presence of 2-cp and 4-cp. A much longer lag phase (19h) was observed with addition of 40mgL-1 2-cp and 100mgL-1 4-cp, comparing with 3 hours when only 200mgL-1 phenol was present. Furthermore, higher concentrations of 2-cp and 4-cp resulted in incomplete transformation: only 80% of initial 100mgL-1 4cp and 50% of initial 40mgL-1 2-cp could be degraded in the presence of 200mgL-1 phenol. Interactions between substrates affected cell growth and substrates degradation significantly and both 2-cp and 4-cp are toxic to the cells. Moreover, 2-cp and 4-cp exhibited different inhibition and toxicity effects. Therefore, besides experimental study of cometabolic transformation of mixtures of phenol, 2-cp and 4-cp, kinetic models for cell growth as well as substrate transformation were developed. The magnitude of the model parameters (K2 = 5.62mgL-1 > K3 = 3.57mgL-1; kd2 = 17.8mgL-1 < kd3 = 51.5mgL-1) provides a quantitative indication of the extent of inhibition and toxicity of phenol and the chlorophenols. The explanations account for these observations were then explored.
URI: http://scholarbank.nus.edu.sg/handle/10635/14638
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Table of Contents.pdf109.33 kBAdobe PDF

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Chapter 1 INTRODUCTION.pdf101.69 kBAdobe PDF

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Chapter 2 LITERATURE REVIEW.pdf205.96 kBAdobe PDF

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Chapter 3 MATERIAL AND METHODS.pdf100.7 kBAdobe PDF

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Chapter 4 RESULTS AND DISCUSSIONS.pdf324.55 kBAdobe PDF

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Chapter 5 CONCLUSIONS AND RECOMMENDATIONS.pdf86.83 kBAdobe PDF

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REFERENCES---1.pdf127.66 kBAdobe PDF

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LIST OF FIGURES.pdf76.04 kBAdobe PDF

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LIST OF TABLES.pdf63.26 kBAdobe PDF

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NOMENCLATURE.pdf60.49 kBAdobe PDF

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SUMMARY.pdf64.05 kBAdobe PDF

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ACKNOWLEDGEMENTS.pdf51.33 kBAdobe PDF

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