Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14635
Title: Isolation and expression analyses of dazl, vasa and sox3 genes in the seabass (Lates calcarifer)
Authors: LIM MENG HUAT
Keywords: seabass, testicular cell culture, sex differentiation , gonadogenesis
Issue Date: 29-Mar-2005
Source: LIM MENG HUAT (2005-03-29). Isolation and expression analyses of dazl, vasa and sox3 genes in the seabass (Lates calcarifer). ScholarBank@NUS Repository.
Abstract: Genes vasa, dazl and sox3 are involved in gonadal development and sex differentiation. The marine seabass is an ideal model for studying these processes because it matures as male and undergoes spontaneous sex reversal to female. This thesis is aimed at making use of this unique organism to study germ cell development and sex differentiation. cDNAs for the above 3 genes were isolated and their RNA expression patterns analysed during embryonic and adult development. Conditions for isolating and culturing seabass testicular cells as an in-vitro model were established. Adult testicular cells can be cultured for up to 40 days. Vasa, dazl and sox3 were transcribed during the culture period, indicating that the cell population comprised of germ cells. These results demonstrate that the cDNAs cloned encode the seabass orthologs for vasa, dazl and sox3, which are all expressed in the gonads. They will provide a useful tool for ongoing experiments to study gonadogenesis and sex differentiation in the seabass as an unique vertebrate model.
URI: http://scholarbank.nus.edu.sg/handle/10635/14635
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Cover page.pdf9.99 kBAdobe PDF

OPEN

NoneView/Download
Content page .pdf40.27 kBAdobe PDF

OPEN

NoneView/Download
thesis content.pdf2.86 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

257
checked on Dec 11, 2017

Download(s)

458
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.