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Title: Aerobiology, image analysis and allergenicity of pollen and spores in Singapore
Keywords: seasonal patterns, diurnal patterns, airspora identification, image analysis, immunoarray, allergy
Issue Date: 10-Mar-2005
Citation: ONG TAN CHING (2005-03-10). Aerobiology, image analysis and allergenicity of pollen and spores in Singapore. ScholarBank@NUS Repository.
Abstract: Allergies to airspora (airborne pollen and spores) are well documented in temperate countries less so in Singapore. To monitor airspora and its associated allergies in Singapore, we developed an optimal method for screening the outdoor airspora samples and observed seasonal and diurnal patterns for all the major airspora types studied as well as correlations with meteorological parameters with seasonal and diurnal patterns. Image analysis coupled with light microscopy could be utilized to identify airspora types and local airspora constituents and closely related pollen types which are morphologically similar can be satisfactorily differentiated. An immunoarray system was developed to simultaneously screen specific IgE molecules to a large panel of allergens. Utilizing the immunoarray to study the prevalence of airspora allergies suggested the presence of airspora types which were not seen in the outdoor samples. Studies carried out have provided useful solutions and answers and suggested more future work.
Appears in Collections:Ph.D Theses (Open)

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