Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14595
Title: A proteomics study of chemically induced cirrhosis in rat liver revealed the mechanism of thioacelamide hepatotoxicity
Authors: LOW TECK YEW
Keywords: Thioacetamide, liver, fibrosis, cirrhosis, proteomics, 2DE-MS
Issue Date: 16-May-2005
Source: LOW TECK YEW (2005-05-16). A proteomics study of chemically induced cirrhosis in rat liver revealed the mechanism of thioacelamide hepatotoxicity. ScholarBank@NUS Repository.
Abstract: A two dimensional gel electrophoresis a?? mass spectrometry (2-DE-MS) approach was applied to analyze the protein expression profiles of livers of rats administered with 300 mg/kg thioacetamide (TAA) for 3, 6 and 10 weeks respectively. 59 protein spots whose expression levels changed significantly upon TAA administration were identified, including 3 novel proteins. These proteins were then sorted according to their biochemical properties and functions. As a result, we found that TAA-administration down-regulated the enzymes of the primary metabolic pathways such as fatty acid I?-oxidation, branched chain amino acids and methionine breakdown. This phenomenon is suggestive of the depletion of succinyl-CoA which affects heme and iron metabolism. Up-regulated proteins, on the other hand, are related to oxidative stress and lipid peroxidation. These proteomics data were corroborated with scientific literature and were integrated into an a??overview modela?? that can now serve as a useful resource for researchers working in this area.
URI: http://scholarbank.nus.edu.sg/handle/10635/14595
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02_Chapter1_Introduction.pdf266.15 kBAdobe PDF

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03_Chapter2_MaterialMethod.pdf102.88 kBAdobe PDF

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04_Chapter3_Results.pdf20.49 MBAdobe PDF

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