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Title: Investigation of the adsorption of biomolecules using surface plasmon fluorescence spectroscopy and microscopy
Keywords: Surface Plasmon, SPFS, SPFM, binding process, fluorescence, evanescent field
Issue Date: 14-Apr-2005
Citation: NIU LIFANG (2005-04-14). Investigation of the adsorption of biomolecules using surface plasmon fluorescence spectroscopy and microscopy. ScholarBank@NUS Repository.
Abstract: On the basis of the investigations in Surface-plamon Field Enhanced Spectroscopy (SPFS), new novel detection schemes for labeled targets were developed. The first one is SPR fluorescence imaging formats. Patterned self assembled monolayers (SAMs) were prepared and used to direct the spatial distribution of biomolecules immobilized on surfaces. The binding processes of labeled target biomolecules from solution to sensor surface were visually and kinetically recorded by the fluorescence microscope, in which fluorescence was excited by the evanescent field of propagating plasmon surface polaritons. The second format which also originates from SPFS technique concerns the coupling of a fluorometry to normal SPR setup. A spectrograph mounted in place of photomultiplier or microscopy can provide the information of fluorescence spectrum as well as fluorescence intensity while fluorescent molecules are excited by the plasmon surface polariton mode.
Appears in Collections:Master's Theses (Open)

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