Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14428
Title: Hydrophobic and hydrophilic substituted polyphosphazenes as scaffolds for liver cell growth
Authors: KO CHOON YING
Keywords: Polyphosphazenes; Scaffold; Hepatocyte; Wettability; Cell viability
Issue Date: 12-Dec-2004
Source: KO CHOON YING (2004-12-12). Hydrophobic and hydrophilic substituted polyphosphazenes as scaffolds for liver cell growth. ScholarBank@NUS Repository.
Abstract: Polyphosphazenes are a broad class of inorganic polymers with the general formula of [NPR]2, with easily modified inorganic or organic side groups. Hence, the versatility of the polymer is high. In this project, Poly[bis(ethyl-glycinato)phosphazene] (PGP), Poly[bis(methylphenoxy)phosphazene] (PMPP) and different copolymer compositions of the poly [(ethyl glycinato)(methylphenoxy)phosphazenes] (PPHOS) were synthesized first from ring opening polymerization of poly(dichlorophosphazene) and then by side group reactions. The polymers were characterized by elemental analysis, FTIR, NMR, DSC, TGA, GPC, SEM, XRD and dynamic water contact angle. The polymers glass transition temperatures are in the range of a??18 to 0oC, and molecular weights in the order of 104 g/mol. GPC results also showed high polydispersity (1-1.5). An increase in the content of the methylphenoxy group resulted in an increase in glass transition temperature and water contact angle. SEM images of the polymers casted as films showed unusual morphologies. In Vitro cytotoxicity testing was done by using L929 cells to examine the biocompatibility of these polymers. The polymer films also were seeded with Hep3B, a cancerous liver cell line, to evaluate the effect of the different chemical functional group and surface wettability on cell growth. Cell proliferation was examined by using MTT assays and two function markers, the secretion of albumin and ethoxyresorufin 0-dealkylase (EROD) activity were also been investigated.
URI: http://scholarbank.nus.edu.sg/handle/10635/14428
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