Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14363
Title: Chitosan: Cellular uptake, cytotoxicity and application in gene delivery
Authors: HUANG MIN
Keywords: chitosan, nanoparticles, FITC, cellular uptake, cytotoxicity, DNA
Issue Date: 6-Oct-2004
Source: HUANG MIN (2004-10-06). Chitosan: Cellular uptake, cytotoxicity and application in gene delivery. ScholarBank@NUS Repository.
Abstract: Chitosan with Mw ranging from 98 to 10 kDa or DD of 61 and 46% were synthesized. and tagged with FITC. Formulation of chitosan into nanoparticles enhanced by up to 1.8 fold its uptake by the A549 cells but did not change the cytotoxicity of the polymer. Cytotoxicity of the chitosan molecules and nanoparticles was attenuated by decreasing DD, but was less affected by a lowering in Mw. A549 uptake of the chitosan nanoparticles was concentration-, time- and temperature-dependent, occurring predominantly by adsorptive endocytosis. Nanoparticle uptake fell by 26% when Mw was decreased from 213 to 10 kDa, and by 41% when DD was lowered from 88 to 46%, the uptake data correlating with the zeta potential of the nanoparticles. Uptake of chitosan molecules did not exhibit saturation kinetics and was less dependent on Mw and DD. The cell associated chitosan molecules were located exptracellularly. Mw and DD of chitosan also affected the cellular uptake of chitosan-DNA nanoparticles and the ability of the chitosan to protect the DNA against nucleases. Lack of protection and poor cellular uptake in turn led to low in vitro transfection efficiency for DNA-complexed nanoparticles prepared with chitosan of low Mw or low DD.
URI: http://scholarbank.nus.edu.sg/handle/10635/14363
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01Cover.pdf18.44 kBAdobe PDF

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02Proceedings.pdf222.51 kBAdobe PDF

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03Chap1-Introduction.pdf242.16 kBAdobe PDF

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04Chap2-Preparation and characterization.pdf183.34 kBAdobe PDF

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05Chap3-Uptake.pdf355.22 kBAdobe PDF

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06Chap4-Effect of Mw and DD.pdf544.67 kBAdobe PDF

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07Chap5-Cytotoxicity.pdf130.39 kBAdobe PDF

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08Chap6-DNA NP.pdf609.33 kBAdobe PDF

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09Chap7-Final conclusion.pdf65.72 kBAdobe PDF

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10Chap8-Future directions.pdf26.69 kBAdobe PDF

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11Reference.pdf345.96 kBAdobe PDF

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12Appendix.pdf214.01 kBAdobe PDF

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