Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/142810
Title: FUNCTIONAL CHARACTERIZATION OF THE ACTIN-BUNDLING PROTEIN PLASTIN IN C. ELEGANS EARLY EMBRYOGENESIS
Authors: DING WEI YUNG
ORCID iD:   orcid.org/0000-0002-2671-0938
Keywords: C. elegans, actin, plastin, embryogenesis, polarization, cytokinesis
Issue Date: 4-Aug-2017
Citation: DING WEI YUNG (2017-08-04). FUNCTIONAL CHARACTERIZATION OF THE ACTIN-BUNDLING PROTEIN PLASTIN IN C. ELEGANS EARLY EMBRYOGENESIS. ScholarBank@NUS Repository.
Abstract: The work presented in this thesis demonstrates that the evolutionarily conserved actin-bundling protein plastin (a.k.a fimbrin) is instrumental for the generation of potent cortical actomyosin contractility in the C. elegans zygote. Endogenous plastin, labelled by GFP was found to be enriched in contractile structures and required for effective coalescence of nascent NMY-2 filaments into large contractile foci and for long-range coordinated contractility in the cortex. In the absence of plastin, polarization and cytokinesis were both significantly compromised, and 50% of embryos died during development. Based on these results as well as in silico CytoSim simulations, we propose a model whereby plastin increased connectivity of cortical F-actin, allowing forces to be transmitted at a much longer length scale, such effective force transmission in turn allows the dynamic reorganization of cortical actomyosin into higher order structures that is critical for robust polarity establishment and timely cell division.
URI: http://scholarbank.nus.edu.sg/handle/10635/142810
Appears in Collections:Ph.D Theses (Open)

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