Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/142743
Title: AN OPTICAL STUDY OF ONGOING ACTIVITY IN THE ZEBRAFISH HABENULA
Authors: MAHATHI RAMASWAMY
Keywords: habenula, spontaneous activity, information processing, zebrafish, GCaMP, CRISPR
Issue Date: 26-Jan-2018
Citation: MAHATHI RAMASWAMY (2018-01-26). AN OPTICAL STUDY OF ONGOING ACTIVITY IN THE ZEBRAFISH HABENULA. ScholarBank@NUS Repository.
Abstract: The brain is constantly active. In sensory regions, this spontaneous activity is proposed to expand dynamic range of information coding by allowing bidirectional responses to signals, or to represent attractors that mirror functional activity. In the habenula, spontaneous activity has been reported to be correlated with odour-evoked activity. Given that the habenula is responsive to several sensory stimuli, this thesis examines the relationship between ongoing activity and visual stimuli. Two photon calcium imaging revealed that onset of light evoked both excitatory and inhibitory responses, while loss of light evoked excitation in a separate population. Cells with higher ongoing activity were not excited by light ON or light OFF. Thus, ongoing activity does not correlate with the response to irradiance change. Cells with correlated activity were, however, grouped together spatially. To test whether gap junctions could underlie this, connexin35.1 was mutated. This did not abolish coupling between cells or ongoing activity. Together, these results suggest that ongoing activity in the habenula of larval zebrafish may expand the dynamic range of response but does not reflect activity evoked by visual stimuli. It is proposed that future studies could examine the possibility that spontaneous activity reflects internal state of the animal.
URI: http://scholarbank.nus.edu.sg/handle/10635/142743
Appears in Collections:Ph.D Theses (Open)

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