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Title: | BIOREMEDIATION OF METALS USING SYNTHETIC ENZYMOLOGY | Authors: | RASHMI RAJASABHAI | Keywords: | gold, MerA, reductase, enzyme engineering, biosensor, golTSB | Issue Date: | 21-Mar-2018 | Citation: | RASHMI RAJASABHAI (2018-03-21). BIOREMEDIATION OF METALS USING SYNTHETIC ENZYMOLOGY. ScholarBank@NUS Repository. | Abstract: | Gold recovery from electronic waste is economically attractive and the use of biological means for recovery is an alternative to current unsustainable practices. As there are no known gold bioreductases, exploiting the substrate promiscuity of mercury reductase (MerA) to target gold presents a viable substitute. Investigation into MerA’s substrate promiscuity revealed that the enzyme not only reduces divalent mercury ions but can also reduce metal ions with other valencies. Key amongst these is the gold trivalent metal ion, Au3+. Reduction of gold by MerA was enhanced up to 15-fold using a combination of enzyme engineering techniques showing that enzymes can be engineered to improve reduction of trivalent metal ions in a typically divalent metal ion reduction system. Using a gold-sensing operon, a biosensor was developed for high-throughput screening where mutant libraries can be screened for increased gold reduction capability. This study highlights the viability of synthetic enzymology for gold bioremediation. | URI: | http://scholarbank.nus.edu.sg/handle/10635/142724 |
Appears in Collections: | Ph.D Theses (Open) |
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